microscale thermophoresis

The Monolith NT.115 Blue/Red uses microscale thermophoresis (MST) technology to measure the interaction between a fluorescently labeled sample and a ligand even under near-native conditions. The interaction is measured in solution (without immobilization) using a temperature gradient over time. The M.O.Control software automatically calculates the binding affinity (KD) from the fitted curves on a plot of fluorescence versus ligand concentration. The Monolith analysis is fast (takes 15 minutes) and requires only small amounts of sample (4 µl per capillary).

The platform includes instruments from NanoTemper Technologies:- Monolith NT.115 Pico-red/Nano-blue instrument for studying the affinity of the protein-ligand system interaction in solution using microscale thermophoresis- Prometheus NT.48 AGOHTU instrument for studying stability and aggregation properties of proteins is used for comprehensive characterization of chemical, thermal in the range of 15 - 110 °C and colloidal stability of proteins in solution by means of precise analysis of the change in internal fluorescence in the structure of the molecule.