LABORATORY OF EXPERIMENTAL REUMATOLOGY AND IMMUNOPATHOLOGY

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Description

Multiparameter assessment of the properties and function of cells of the immune system:

Surface staining of phenotypes (markers on cells and intracellular antigens) by the technique of multi-parameter flow cytometry - work with freshly isolated lymphocytes and blood monocytes, cultured cells (including cell lines).

Functional tests:
a) lymphocytes isolated from blood:
- ability to proliferation
- susceptibility to apoptosis - measurement of changes in mitochondrial potential, assessment of surface phosphatidylserine expression,
- lymphocyte T cytotoxicity and intracellular caspase activity in living cells,
- evaluation of the intracellular activity of calpain proteases,
b) monocytes, granulocytes, macrophages:
- measurement of the ability to phagocytosis and oxygen burst.

Tests in body fluids of secreted cytokines and other active proteins using the CBA method (quantitative flow cytometry) enabling the simultaneous measurement of up to 6 factors in 50 µl of material (material and cost savings compared to the ELISA technique, while maintaining measurement accuracy and assessment of absolute concentration).

MAIN EQUIPMENT:
- flow cytometers: FACScan ™, FACSArray ™,
- cell sorters: FACSAria III ™ and FACSVerse ™ (Becton Dickinson)
- high-quality Nikon Eclipse Ti-E universal fluorescence microscope

Examples of research articles:
Publications

Dendritic cells' characteristics in patients with treated systemic lupus erythematosus - ANNA WARDOWSKA, ŻANETA SMOLEŃSKA, KATARZYNA A. LISOWSKA, ZBIGNIEW ZDROJEWSKI, MICHAŁ PIKUŁA. - Acta Biochim. Pol. 2020; vol. 67, nr 3, s. 417-429, bibliogr. [52] poz., streszcz. ang.
Alterations in peripheral blood B cells in systemic lupus erythematosus patients with renal insufficiency - ANNA WARDOWSKA, MICHAŁ KOMORNICZAK, ANETA SKONIECKA, BARBARA BUŁŁO-PIONTECKA, KATARZYNA A. LISOWSKA (fizjol.), M. ALICJA DĘBSKA-ŚLIZIEŃ, MICHAŁ PIKUŁA. - Int. Immunopharmacol. 2020; vol. 83, art. ID 106451, s. 1-13, bibliogr. 73 poz., streszcz. ang.
Enhancement of circulating and intestinal T regulatory cells and their expression of helios and Neuropilin-1 in children with inflammatory bowel disease - KATARZYNA SZNURKOWSKA, JUSTYNA LUTY, EWA BRYL, JACEK M. WITKOWSKI, Blanka Hermann-Okoniewska, PIOTR LANDOWSKI, MARTA KOSEK, AGNIESZKA SZLAGATYS-SIDORKIEWICZ. - J. Inflamm. Res. 2020, vol.13, s. 995-1005, bibliogr. 42., streszcz. ang.

Details

Type of infrastructure:
'Core Lab' laboratory
Strategic infrastructure:
Yes
Accessibility
commercial activities
scientific research

Manager / operator

Manager

Manager's name
Prof. Jacek M. Witkowski, Ph.D.
Manager's e-mail
jacek.witkowski@gumed.edu.pl
Manager's phone
58 349 15 10

Operator

Operator's name
Prof. Jacek M. Witkowski, Ph.D.
Operator's e-mail
jacek.witkowski@gumed.edu.pl
Operator's phone
58 349 15 10

Laboratory where the equipment is located

Name of the laboratory
LABORATORY OF EXPERIMENTAL REUMATOLOGY AND IMMUNOPATHOLOGY

Location

University
Medical University of Gdańsk
Organisational Unit
Faculty of Medicine, Department of Physiopathology, LABORATORY OF EXPERIMENTAL REUMATOLOGY AND IMMUNOPATHOLOGY
Street
Dębinki 7
Postal Code
80-211
City
Gdańsk

Access rules

Access rules

Both scientific cooperation and commercial use is possible, in accordance with Rules of CORE FACILITY labs and access is determined separately for each project in accordance with its specificity.