iBlot 2 Dry Blotting System

Description

The iBlot™ 2 Dry Blotting System is a fast, convenient, and buffer-free system for electrotransferring proteins from mini, midi, and E-PAGE™ gels to nitrocellulose or PVDF membranes. The device utilizes dry transfer technology, eliminating the need for traditional buffer solutions and wet transfer equipment.

Examples of research articles:
Publications

1. Kobiela A, Hovhannisyan L, Jurkowska P, de la Serna JB, Bogucka A, Deptuła M, Paul AA, Panek K, Czechowska E, Rychłowski M, Królicka A, Zieliński J, Gabrielsson S, Pikuła M, Trzeciak M, Ogg GS, Gutowska-Owsiak D. Excess filaggrin in keratinocytes is removed by extracellular vesicles to prevent premature death and this mechanism can be hijacked by Staphylococcus aureus in a TLR2-dependent fashion. J Extracell Vesicles. 2023 Jun;12(6):e12335. doi: 10.1002/jev2.12335. PMID: 37338870; PMCID: PMC10281372. 2. Kobiela A, Frackowiak JE, Biernacka A, Hovhannisyan L, Bogucka AE, Panek K, Paul AA, Lukomska J, Wang X, Giannoulatou E, Krolicka A, Zielinski J, Deptula M, Pikula M, Gabrielsson S, Ogg GS, Gutowska-Owsiak D. Exposure of Keratinocytes to Candida Albicans in the Context of Atopic Milieu Induces Changes in the Surface Glycosylation Pattern of Small Extracellular Vesicles to Enhance Their Propensity to Interact With Inhibitory Siglec Receptors. Front Immunol. 2022 Jun 9;13:884530. doi: 10.3389/fimmu.2022.884530. PMID: 35784319; PMCID: PMC9248261.

Details

Type of infrastructure:
large research equipment
Strategic infrastructure:
No
Accessibility
internal

Manager / operator

Manager

Manager's name
prof. Danuta Gutowska-Owsiak
Manager's e-mail
danuta.gutowska-owsiak@ug.edu.pl
Manager's phone
+48 58 523 63 09

Operator

Operator's name
prof. Danuta Gutowska-Owsiak
Operator's e-mail
danuta.gutowska-owsiak@ug.edu.pl
Operator's phone
+48 58 523 63 09

Manufacturer

Manufacturer
Thermo Fisher Scientific Inc

Laboratory where the equipment is located

Name of the laboratory
Experimental and Translational Immunology Group

Location

University
University of Gdańsk
Organisational Unit
Intercollegiate Faculty of Biotechnology
Street
Abrahama 58
Postal Code
80-307
City
Gdańsk
Room
168D