Digitized system for amplification of nucleic acids
An automated digital emulsion PCR system (BioRad QX200) using digital droplet PCR (ddPCR) is used to amplify nucleic acids in an emulsion system with simultaneous quantitative evaluation of the obtained product. The advantage of the ddPCR method is its high sensitivity and, above all, in comparison to the Real-time PCR technique, the lack of necessity to refer to standard curves or reference gene.