Ultra High Performance Liquid Chromatography coupled with quadrupole and time of flight mass spectrometry detection (UHPLC-QTOF/MS)
Liquid chromatography 1260 Infinity, coupled with mass spectrometer with a QqQ analyzer, model 6430 LC-QqQ/MS, Agilent Technologies. It is a powerful tool for targeted metabolomics analysis including screening and quantification of known metabolites. This technique allows the detection and quantification of specific molecules in a biological complex mixture (e.g. urine, plasma, serum, saliva), isotopically labeled internal standards are often used for accurate quantification.