HPLC

Agilent Technologies HPLC system (model Agilent 1200) with dual pump, degasser, autosampler, column thermostat, and diode array detector Spectrometer HCT Ultra from Bruker Daltonics with ion trap analyzer. The mass spectrometer is equipped with three types of ion sources using different ways to ionize the sample: electrospraying (sources: ESI and off-line nano ESI) and chemical ionization (APCI). Possibility to perform MS(n) analyses. The set is dedicated to determining the mass and structure of synthesized compounds, qualitative and quantitative analysis of composition of mixtures.

The laboratory infrastructure includes 1. Shimadzu high-performance liquid chromatograph equipped with an automatic sample feeder, a diode array detector, a pump and a thermostat; 2. Shimadzu High Performance Liquid Chromatograph equipped with an automatic sample feeder, a diode array detector, a pump and a thermostat ; 3. Shimadzu Ultra Performance Liquid Chromatograph equipped with an automatic sample feeder, a diode array detector, a pump and a thermostat.

The set consists of an Agilent 1200 liquid chromatograph (Agilent Technologies, Waldboronn, Germany) with a tandem mass spectrometer (QTRAP5500, Applied Biosystems, Sciex; Concorde, ON, Canada) equipped with a triple quadrupole and a linear ion trap.

The QTRAP spectrometer is equipped with a triple quadrupole, in which the second analyzer can work interchangeably as a quadrupole and an ion trap. It is therefore characterized by high sensitivity and enables operation in the following modes: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, MRM3, TripleTrap Scanning. Perfect for metabolite identification, MRM based quantification. It provides excellent selectivity and measurement speed. In the case of this spectrometer, it is possible to record MS3 spectra for i.a. quantitative analyzes.

The laboratory is equipped with three AB Sciex mass spectrometers: MALDI TOF / TOF 5800, TripleTOF 5600+, QTRAP 6500, as well as the CESI 8000 Plus (Sciex) capillary electrophoresis system.